Reporter

Part:BBa_K2271023:Design

Designed by: Remy Bastiaan Tjeng   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)


roGFP2: redox sesning green flourescent protein for yeast expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

roGFP2 was optimized for the expression in yeast. This part enables several fusions, with either signaling tags in order to achieve a specific localization inside the cells or proteins in order to enhance or specify the redox sensing. For example the fusion with Glutaredoxin, is reported to enhance the speed of sensing in vivo redox changes of the glutathione pool Schwarzländer et al. (2015)

Source

Synthese from IDT. DNA sequence from Gutscher, M., et al. (2008). "Real-time imaging of the intracellular glutathione redox potential." Nat Methods 5(6): 553-559. Yeast codon optimized

References